WFHSS Questions and Answers - Question Q01317

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WFHSS - Questions & Answers - Quality Assurance - Q01317
BI Test

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From: (Malaysia) Date: 12 October 2006, 04:44 [GMT]
Subject: BI Test

What is the diffrent between Rapid BI test & BI test ?

From: (Israel) Date: 14 October 2006, 17:23 [GMT]
Subject: Re: BI Test

Dear Hairul,
Biological indicators rely of pH indicator to measure production of acid metabolism in the growth medium. Rapid readout BIs detect active - spore associated enzyme, if it survives the sterilization process.
Regards,
Yaffa Raz

From: Date: 16 October 2006, 23:30 [GMT]
Subject: Re: BI Test

Good day Hairul,
As Yaffa correctly indicates, the self contained BIs undergo a color change when the growing microbes change the pH of the growth media. At least one of the Rapid Readout BI's allow an additional test. A fluorescein coupled sugar is added to the growth media. If a microbe eats even one piece of the treated sugar, the fluorescein is released into the media and will glow when exposed to ultraviolet (black) light. Special readers can detect this glow before the human eye can detect the color change caused by the change of pH in the growth media.
If you do not use the special reader, the Rapid Readout BIs can be used like the regular BIs, they just cost more.
Warm Regards,
Pete Bobb

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